
Use of Fresh (Non-cryopreserved) Bone Marrow in Autologous BMT
Certain conditioning regimens, e.g., single dose melphalan, permit the possibility of taking a bone marrow harvest immediately prior to therapy, storing it fresh at 4oC and infusing it after the conditioning therapy.
Such treatments may be suitable for patients with myeloma and neuroblastoma.
- The marrow harvest bag should be stored at 4oC and a maximum storage period of 48 hours should be planned prior to reinfusion.
- The product should be gently mixed after 24 hours.
Overnight Storage of PBSC Harvest
- Product nucleated cell count will be adjusted to 1 x 108/ml using autologous plasma.
- The product will be transferred to a gas permeable bag for storage.
- The product will be stored overnight in a continuously monitored cold room at 4°C.
- Continuous agitation is not necessary.
- Product may be stored up to 48 hours under the above conditions if necessary although the product will need to be mixed by hand after 24 hours.
Topic Code: 9014